Part:BBa_K1647011
Lac promoter+taRNA
This part is composed of a IPTG induced lac promoter, a taRNA sequence, an a terminator. Its function is to generate taRNA which can unlock the CrRNA by competing with the RBS under the introduction of IPTG. Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a biological reagent which is a molecular mimic of allolactose and triggers transcription of the lac operon and subsequent gene expression. The lac repressor, LacI, is also caused to dissociate from the promoter gene when lactose or allolactose-like module is present. LacI also binds to the major groove in the operator sequence and blocks T7 RNA polymerase from binding the promoter sequence. IPTG is therefore required in order to enable the binding and action of T7 RNA polymerase to transcribe the plasmid, or DNA fragment with T7 promoter. When IPTG exists, the lac promoter is activated ,and then taRNA is expressed, taRNA is a short sequence which can act as the “key” to riboregulator when transcripted into mRNA, so it can pair with a spectacular riboregulator, and unlock it , making it non-functional.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 206
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